The results showed that the analysis can be performed at 499 nm, using ethanol as acridine orange (AO) solvent, the mole ratio of dodecylbenzenesulfonate (DBS) acridine orange (AO) 2:1, and pH 2.97. The analysis of sodium dodecylbenzenesulfonate (SDBS) is based on the formation of ion pair between dodecylbenzenesulfonate (DBS) and acridine orange (AO). This study was divided into two steps, isolation of sodium dodecyl benzene sulfonate (SDBS) in the catfish with soxhlet extraction and the analysis of SDBS. This research aims to determine the optimum conditions of analysis and to determine validation parameters of sodium dodecylbenzenesulfonate (SDBS) analysis in a catfish.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).The analytical method development on an anionic surfactant of sodium dodecylbenzenesulfonate (SDBS) in a catfish using spectrophotometer UV-Vis using acridine orange (AO) has been conducted.

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